

If health impacts of fine particles (PM 2.5) from diesel combustion are well documented (Gangwar et al., 2012), those from biomass combustion need substantial information and improvements. Among these energies, biomass production and utilization is growing considerably since it offers the possibility to provide partial substitution of fossil fuels. Renewable energies are destined to play a very important role in the future world energy balance. In order to approach Beer-Lambert law, the linear correlations of fluorescence and chemical content in this paper consider fluorophores as carbonaceous content of dust like residual carbon, WSOC, HULIS and. Fluorescence microscopy allows to obtain the fluorescence emission within relatively cheap and fast method this propriety of particles could lead to characterize some chemical content of dust. SEM can also be coupled with EDS spectra in order to have information on atomic composition of fine particles, nevertheless, it is also a cost-effective and time-consuming method and no information about carbonaceous molecules is obtained. SEM observations enabled to have very good morphological information about PM2.5. Also, in order to remove aggregation during impaction, particles have been recovered from impactor plate, re-suspended in isopropanol, mixed using sonication method, quickly dried, and observed. This agglomeration is hard to break as shown in the images from re-suspension ( Fig. Even after only 20 seconds of impaction and not directly close to impaction spots, the particles tend to agglomerate. They were made on PM 2.5-1 and PM 1-0.4 during miscanthus combustion at laboratory scale.
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